| Location | No. of seeds
(weight grams) | Number
of plants | Date
collected | Collection number
Collection location | Date
stored | % Viability | Storage
temperature | | BGA | 34,000 (3.48 g) | 50+ | 28-Feb-2017 | DJD3621
Murray | 1-Nov-2017 | 85% | +5°C, -18°C, -80°C |
Location: BGA — the seeds are stored at the Adelaide Botanic Gardens, MSB — the seeds are stored at the Millennium Seed Bank, Kew, England.
Number of plants: This is the number of plants from which the seeds were collected.
Collection location: The Herbarium of South Australia's region name.
% Viability: Percentage of filled healthy seeds determined by a cut test or x-ray.
| Date | Result | T0 | T50 | Pre-treatment | Germination medium | Incubator: Photoperiod / Thermoperiod |
| Jun-17 | 100% | 7 | 7 |
seeds leached in water for 24 h;
1% agar;
Incubated under spring/autumn conditions |
| Jun-17 | 100% | 7 | 7 |
500 mg/L gibberellic acid for 24 h;
1% agar;
Incubated under spring/autumn conditions |
| Jun-17 | 96% | 7 | 7 |
500 mg/L gibberellic acid for 24 h;
1% agar;
Incubated under winter conditions |
| Jun-17 | 30% | 14 | NA |
seeds leached in water for 24 h;
1% agar;
Incubated under winter conditions |
Result: Maximum percentage of germination observed.
T0: Number of days before first germinant observed.
T50: Number of days to achieve 50% germination.
Pre-treatment: The initial treatment that the seeds received prior to placement on germination media.
Germination medium: The substrate that seeds were placed on for the duration of the germination experiment.
Incubator conditions:
Photoperiod: The duration of light exposure that the seeds were subject to during a 24 hour period.
Thermoperiod: The constant or diurnal temperatures that seeds were subject to during a 24 hour period.
Winter conditions: 15°C 20 h (3am→11pm); 5°C 4 h (11pm→3am) / 10 h light (8am→6pm); 14 h dark (6pm→8am)
Spring/Autumn conditions: 22°C 12 h (8am→8pm); 10°C 12 h (8pm→8am) / 12 h light (8am→8pm); 12 h dark (8pm→8am)
Summer conditions: 30°C 14 h (6am→8pm); 15°C 10 h (8pm→6am) / 14 h light (6am→8pm); 10 h dark (8pm→6am)
