Location | No. of seeds (weight grams) | Number of plants | Date collected | Collection number Collection location | Date stored | % Viability | Storage temperature | BGA MSB | 2,300 (2.75 g) 2,300 (2.75 g) | 40 | 26-Oct-2004 | DJD22 Gairdner-Torrens | 28-Mar-2006 | 100% | -18°C |
BGA MSB | 7,200 (8 g) 2,000 (2.22 g) | 100+ | 5-Oct-2010 | DJD1872 Lake Eyre | 1-Jan-2012 | 85% | -18°C |
BGA MSB | 1,800 (2.78 g) 1,800 (2.78 g) | 100+ | 2-Nov-2013 | DJD2799 Lake Eyre | 24-Mar-2015 | 100% | -18°C |
Location: BGA — the seeds are stored at the Adelaide Botanic Gardens, MSB — the seeds are stored at the Millennium Seed Bank, Kew, England.
Number of plants: This is the number of plants from which the seeds were collected.
Collection location: The Herbarium of South Australia's region name.
% Viability: Percentage of filled healthy seeds determined by a cut test or x-ray.
Date | Result | T0 | T50 | Pre-treatment | Germination medium | Incubator: Photoperiod / Thermoperiod |
Oct-14 | 93% | 9 | 9 |
500 mg/L gibberellic acid 48 h; 1% agar; Incubated under winter conditions |
Sep-14 | 72% | 9 | 9 |
leached in water 4 d, 500 mg/L gibberellic acid 48 h; 1% agar; Incubated under winter conditions |
Oct-14 | 60% | 9 | 9 |
leached in water 4 d; 1% agar; Incubated under winter conditions |
Result: Maximum percentage of germination observed.
T0: Number of days before first germinant observed.
T50: Number of days to achieve 50% germination.
Pre-treatment: The initial treatment that the seeds received prior to placement on germination media.
Germination medium: The substrate that seeds were placed on for the duration of the germination experiment.
Incubator conditions:
Photoperiod: The duration of light exposure that the seeds were subject to during a 24 hour period.
Thermoperiod: The constant or diurnal temperatures that seeds were subject to during a 24 hour period.
Winter conditions: 15°C 20 h (3am→11pm); 5°C 4 h (11pm→3am) / 10 h light (8am→6pm); 14 h dark (6pm→8am)
Spring/Autumn conditions: 22°C 12 h (8am→8pm); 10°C 12 h (8pm→8am) / 12 h light (8am→8pm); 12 h dark (8pm→8am)
Summer conditions: 30°C 14 h (6am→8pm); 15°C 10 h (8pm→6am) / 14 h light (6am→8pm); 10 h dark (8pm→6am)