Nicotiana burbidgeae

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Adelaide

Arkaroola

Ceduna

Coober Pedy

Hawker

Innamincka

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Nicotiana ,named after Jean Nicot (1530-1600), a French Ambassador for the King of France to Lisbon in 1560, who sent the first tobacco plant to France. Burbidgeae named after Miss Nancy Tyson Burbidge (1912-1977), for her contributions to taxonomic botany in Australia.

Endemic to South Australia and found only in the south-east end of the Dalhousie Springs complex in Witjira National Park, growing along arid creeklines that may be sub-saline and gypseous. Native. Rare in South Australia.
Herbarium region: Lake Eyre
NRM region: South Australian Arid Lands
AVH map: SA distribution map (external link)

Erect leafy annual or short lived perennial herb to at 75 cm high; covered in hairs and with several branches near base. Leaves mostly cauline; basal ones to 10 cm long and 7 cm wide; oval with apex obtuse; base broadly attached; leaves of the middle stem region smaller, rather more obovate; gradually decreasing in size upwards with all, rather thick and relatively fleshy. Inflorescence of solitary white tubular flowers is distributed along upper parts of leafy stems. Flowering between June and September. Fruits are papery brown, ovate capsule to 12 mm long, about equal to enveloping calyx; splitting by 2 major and 2 minor fissures. Seeds are dark brown reniform seed to 1.2 mm long and 0.8 mm wide, covered in a net-like surface. Seed embryo type is linear, fully-developed.

Collect seeds between September and December. Collect mature capsules that are brown or turning a pale yellow straw-colour and contain brown seeds. Can collect individual capsules or break off the whole fruit spike. Capsules that are open may still contain a good quantity of seeds. Place the capsules in a tray and leave to dry for one to two weeks, then rub the capsules gently by hand to dislodge the seeds. Use a sieve to separate the unwanted material. Store the seeds with a desiccant such as dried silica beads or dry rice, in an air tight container in a cool and dry place. From three collections, the seed viability was high, ranging form 90% to 100%.

Location	No. of seeds (weight grams)	Number of plants	Date collected	Collection number Collection location	Date stored	% Viability	Storage temperature
BGA	41,000 (9.92 g)	30+	3-Sep-2015	DJD3167 Lake Eyre	2-May-2017	100%	+5°C, -18°C, -80°C
BGA MSB	58,000 (13.95 g) 58,000 (13.95 g)	50+	1-Sep-2015	TST1208 Lake Eyre	2-May-2017	90%	+5°C, -18°C, -80°C
BGA	188,000 (48.54 g)	50+	20-Sep-2016	DJD3437 Lake Eyre	1-Nov-2017	100%	+5°C, -18°C, -80°C
BGA	57,250 (14.1 g)	5	15-Jan-2020	TST1443 Lake Eyre	24-Jun-2020	100%	-18°C, -80°C

Location: BGA — the seeds are stored at the Adelaide Botanic Gardens, MSB — the seeds are stored at the Millennium Seed Bank, Kew, England.
Number of plants: This is the number of plants from which the seeds were collected.
Collection location: The Herbarium of South Australia's region name.
% Viability: Percentage of filled healthy seeds determined by a cut test or x-ray.

Date	Result	T0	T50	Pre-treatment \| Germination medium \| Incubator: Photoperiod / Thermoperiod
Jul-17	96%	14	14	500 mg/L gibberellic acid for 24 h; 1% agar; Incubated under winter conditions
Jul-17	92%	14	14	500 mg/L gibberellic acid for 24 h; 1% agar; Incubated under spring/autumn conditions
Jul-16	73%	7	7	500 mg/L gibberellic acid 20 h; 1% agar; Incubated under spring/autumn conditions
Jul-16	43%	7	NA	500 mg/L gibberellic acid 20 h; 1% agar; Incubated under winter conditions
Jul-17	18%	14	NA	1% agar; Incubated under spring/autumn conditions
Jul-17	14%	14	NA	1% agar; Incubated under winter conditions

Result: Maximum percentage of germination observed.
T0: Number of days before first germinant observed.
T50: Number of days to achieve 50% germination.
Pre-treatment: The initial treatment that the seeds received prior to placement on germination media.
Germination medium: The substrate that seeds were placed on for the duration of the germination experiment.
Incubator conditions:
Photoperiod: The duration of light exposure that the seeds were subject to during a 24 hour period.
Thermoperiod: The constant or diurnal temperatures that seeds were subject to during a 24 hour period.
Winter conditions: 15°C 20 h (3am→11pm); 5°C 4 h (11pm→3am) / 10 h light (8am→6pm); 14 h dark (6pm→8am)
Spring/Autumn conditions: 22°C 12 h (8am→8pm); 10°C 12 h (8pm→8am) / 12 h light (8am→8pm); 12 h dark (8pm→8am)
Summer conditions: 30°C 14 h (6am→8pm); 15°C 10 h (8pm→6am) / 14 h light (6am→8pm); 10 h dark (8pm→6am)