Nicotiana sp. Corunna (D.E.Symon 17088)

Adelaide

Arkaroola

Ceduna

Coober Pedy

Hawker

Innamincka

Marla

Marree

Mount Gambier

Oodnadatta

Renmark

Wudinna

Keith

Yunta

Nicotiana, named after Jean Nicot (1530-1600), a French Ambassador for the King of France to Lisbon in 1560, who sent the first tobacco plant to France. Corunna, named after Corunna Station, north of Iron Knob in South Australia, where the species was first collected.

Endemic to South Australia and found in a few locations around Port Augusta, growing in sandy or stony loamy soils in creeklines and hillsides. Native. Rare in South Australia.
Herbarium region: Eyre Peninsula
NRM region: Eyre Peninsula
AVH map: SA distribution map (external link)

Erect herb to 1 m tall with more or less glabrous stems and leaves. Leaves radical and cauline; mostly lanceolate. Inflorescence erect, few-branched spike with tubular flowers, white inside and cream, green or purple-tinged outside. Fruits are brown ellipsoid to ovoid capsule. Seeds are brown reniform seed to 0.7 mm long and 0.5 mm wide, covered in wrinkles. Seed embryo type is linear, fully-developed.

Collect seeds between October and December. Collect mature capsules, those that are brown or turning a pale straw-colour and contain brown seeds. Can collect individual capsules or break off the whole fruit spike. Place the capsules in a tray and leave to dry for one to two weeks, then rub the capsules gently by hand to dislodge the seeds. Use a sieve to separate the unwanted material. Store the seeds with a desiccant such as dried silica beads or dry rice, in an air tight container in a cool and dry place.

Location	No. of seeds (weight grams)	Number of plants	Date collected	Collection number Collection location	Date stored	% Viability	Storage temperature
BGA	11,900 (1.44 g)	6	1-Nov-2004	DES17088 Eyre Peninsula	1-Jan-2016	95%	-18°C

Location: BGA — the seeds are stored at the Adelaide Botanic Gardens, MSB — the seeds are stored at the Millennium Seed Bank, Kew, England.
Number of plants: This is the number of plants from which the seeds were collected.
Collection location: The Herbarium of South Australia's region name.
% Viability: Percentage of filled healthy seeds determined by a cut test or x-ray.

Date	Result	T0	T50	Pre-treatment \| Germination medium \| Incubator: Photoperiod / Thermoperiod
Aug-15	90%	7	7	500 mg/L gibberellic acid 48 h; 1% agar; Incubated under spring/autumn conditions
Aug-15	88%	7	7	1% agar; Incubated under spring/autumn conditions
Aug-15	82%	7	7	500 mg/L gibberellic acid 48 h; 1% agar; Incubated under winter conditions
Aug-15	44%	7	NA	1% agar; Incubated under winter conditions

Result: Maximum percentage of germination observed.
T0: Number of days before first germinant observed.
T50: Number of days to achieve 50% germination.
Pre-treatment: The initial treatment that the seeds received prior to placement on germination media.
Germination medium: The substrate that seeds were placed on for the duration of the germination experiment.
Incubator conditions:
Photoperiod: The duration of light exposure that the seeds were subject to during a 24 hour period.
Thermoperiod: The constant or diurnal temperatures that seeds were subject to during a 24 hour period.
Winter conditions: 15°C 20 h (3am→11pm); 5°C 4 h (11pm→3am) / 10 h light (8am→6pm); 14 h dark (6pm→8am)
Spring/Autumn conditions: 22°C 12 h (8am→8pm); 10°C 12 h (8pm→8am) / 12 h light (8am→8pm); 12 h dark (8pm→8am)
Summer conditions: 30°C 14 h (6am→8pm); 15°C 10 h (8pm→6am) / 14 h light (6am→8pm); 10 h dark (8pm→6am)